Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6’s role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1 and its activation of IL-1ra and IL-10. There is some early evidence that IL-6 can be used as an inflammatory marker for severe COVID-19 infection with poor prognosis, in the context of the wider coronavirus pandemic.

Human IL-6 ELISA Kit Principle

This Human IL-6 ELISA Kit is based on sandwich ELISA, the IL-6 specific monoclonal antibody was pre-coated in the microwells, the IL-6 in sample or the IL-6 standard will be captured by the coated antibody, after wash step, the unbonded IL-6 will be removed, and then another IL-6 specific monoclonal antibody is added and incubated. After then, the plate is washed again, and the substrate is added. The color of the wells is positively proportional to the IL-6 in it. After adding the stop solution, OD values are measured with ELISA reader. Unknown samples can be calculated from the standard curve.

Kit Components

This Human IL-6 ELISA kit is a readily commercial product, it has the following components, which is sufficient for 42 testing in duplicated wells. Please always check the kit instruction manual for packaging details.

• Microtiter plate, 96wells,
• Standard, will be diluted to 7 vials,
• HRP conjugate, 1 vial,
• TMB substrate, 1 vial,
• Assay buffer, 1 vial,
• Wash buffer, 1 vial,
• Sealer film or plate cover, 1p pc
• Stop solution, 1 vial,

Specificity and sensitivity

The sensitivity is 0.26 pg/mL as MDD. The specificity is over 99%.

Result determination

The sample OD value is measured by an ELISA reader, then the standard curve is plotted. We recommend 4PL regression model or another equivalent model.

Storage and expiry

2-8 degree Celsius for at least 12 months.

Caution

• This kit is for research use only.
• The standard solution in the kit is human IL-6.
• Please read the kit instruction manual carefully before testing.
• Each run will take 80min or more, please schedule your time before experiment.