Choline oxidase catalyzes the four-electron, two-step, flavin-mediated oxidation of choline to glycine betaine. The enzyme is important both for medical and biotechnological reasons, because glycine betaine is one among a limited number of compatible solutes used by cells to counteract osmotic pressure. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donors with oxygen as acceptor. The systematic name of this enzyme class is choline: oxygen 1-oxidoreductase. This enzyme participates in glycine, serine, and threonine metabolism. It employs one cofactor, FAD.

Specificity and sensitivity

According to internal validation, this enzyme can be used in the colorimetric enzymatic assay for the determination of choline in baby formula and other dairy products.  Contact us for the details of this commercial kit.

Storage and expiry

-20 degree Celsius for at least 24 months.

Caution

• This product is for research use only.
• Final dilution shall be determined by the user according to the specific experiment design.

Reference

• Woollard, D. C., & Indyk, H. E. (2000). Determination of choline in milk and infant formulas by enzymatic analysis: collaborative study. Journal of AOAC International, 83(1), 131–138.
• hillips, M.M. Analytical approaches to determination of total choline in foods and dietary supplements. Anal Bioanal Chem 403, 2103–2112 (2012). https://doi.org/10.1007/s00216-011-5652-5